Thursday, December 18, 2014

Human uniprot FASTA database with the PRTC peptides

A coworker in my day job and I generated a FASTA database today that I think some of you might find useful out there.  It is simply the Uniprot Human database (a few months old) with the PRTC peptides added to the database.  This would be useful, for example, if you wanted to see how low in concentration you could still reliably sequence peptides.

You can download it here (Uniprot_Human_plus_PRTC).

If you were going to search this directly you would need these dynamic modifications K, SILAC labeled + 8 and R, SILAC labeled +10.  You would also need to run your engine with at least 1 allowed missed cleavage, since one of the synthetic peptides has a K in the middle of it.

I'm not guaranteeing there are no typos, but I did try!

1 comment:

  1. Do you happen to have this file at a new link? The link above doesn't work for me.