Saturday, February 28, 2026

Proteomic analysis of postbiotic inhibition of Salmonella typhimurium!

 


Borrowed this picture from the MicroChem website while I was looking up what a Kirby-Bauer Inhibition assay was. Its a microbial zone of inhibition assay! 

Paper link!



What's a postbiotic? It's a metabolic secondary product of "good" bacteria or other microorganisms. So the question was something like "what would inhibit Salmonella typhimurium" and when we see that inhibition, what is causing that?

And since Salmonella is not a fun thing for humans to get, the goal is to find compounds that will inhibit it to limit infections. 

Interestingly the proteomics from these researchers was performed by sending the samples to the University of Copenhagen Chemistry department where they digested them and did peptide mass fingerprinting by MALDI-TOF. I suspect microbiologists are so used to doing everything with lasers that this is due to the contacts they generally have. The authors come up with a solid table of both small molecules identified by GCMS and proteins identified by these methods that may be next line inhibition chemicals for this nasty bacterium.

As an aside I sincerely hope that these authors are safe and continue to remain so in the wake of the blatantly illegal actions of an 800 year old narcoleptic dictator that began a few hours ago. 

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