As my very first takeaway from ASMS2025,
I'd like to start with this paper that is just a little bit older than I thought it was when it came up in my talk on Monday.
Oh man, did we ever see some awful chromatography this week. Whole proteomics on 5cm columns was often higher resolution LC separations. There are 4cm columns and probably shorter. And often at low pressure. Now, maybe all the hardware advances that have happened on the gas phase should negate the statements in this commentary? But I'm skeptical that is truly the case. And maybe I'm just old and old fashioned, but proteomics did seem to be the one place where the LC seemed to be left the most behind. I popped in on some food chemistry talks and a couple environmental and - they also have big complex matrices - and they were still leaning on UHPLC - often with very fast gradients and the discrepancy was a little jarring.
Time will tell, I guess! I just wanted to drop this overall impression while I had a second - not long enough to write anything more involved.
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