Crosslinking proteomics is not really my thing. I've done it a few times over the years and I've personally had success with in vitro experiments with a few proteins at a time (metric of success being happy collaborator).
I really like the tone of this manuscript which introduces a new crosslinker. From the very beginning the tone feels like "crosslinking mass spectrometry is still hard. And this is a new way of doing it. It's STILL hard, and could be improved, so here are ALL of our notes."
It feels very honest and open all the way through - from how to make it through how it compares to the other stuff -through 3 different mechanisms of enriching these crosslinked peptides.
If I had to do this I don't think there could be anything more helpful than - the exact masses observed for the fragments AND their delta masses. And they're all helpfully provided directly in the text!
I feel like this is a great example that maybe we don't have to completely oversell every scientific advancement that goes very counter- to what we often read (or may be expected to write?) Cool science. Better writing. 100% recommended.
No comments:
Post a Comment