Wow - this is just another stunning example of spatial proteomics and what it can do!
Highlights?
The cuts are made with a little Leica LMD7 system which can drop cuts directly into 96 or (as used here) 384 well plates. These smaller systems appear a bit more approachable, but might not have the resolution to do some of the really precise cuts (?) I'm not sure.
Here the regions that were excised were in the 50-100 cell range and guided by histological stains (I think? not my field and I can't spend all morning on it).
Cells were digested in plate - ACN first to lyse - that's dried off then LysC then trypsin.
The cleanup was done on EvoTips and analysis was on a TIMSTOF Ultra(? I forget, but pretty sure) using the EvoSep 30SPD method.
Analysis with DIA-NN allowed them to quantify 10,000 protein groups?? From 50-100 cell cuts! Just stunning work I can't wait to circulate to my colleagues this morning!
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