An update on the proteomics of extracellular vesicles!

 

Are you also confused by what an extracellular vesicle is? And or why people want you to do proteomics on them? Did you get two collaboration requests this week to work on them while also remembering that you already ran 400 for someone and you aren't entirely sure where you put the .d files that you are supposed to be using to write a grant. 

This isn't me I'm talking about. There is no way a single lab move would make me so disorganized to have 20 cases of p20 tips and no way to pipette a larger volume without adding to a large list on my tablet that says "I owe Matt MacDonald lab..." And I absolutely have every file and every file backed up. It just might be in the hard drives with the p200 tips that I absolutely have on a shelf...somewhere.... 

To whoever you are you hypothetical person who needs to know about EVs I present this really nice and succint and new review - 

OH! And this is important! I just found this out and you guys should know this. 

There is a Qiagen kit out there for EV enrichment. The buffer that they use in the final step to lyse the EVs contains Triton X-100. I got suspicious when I checked the MSDS and it was essentially a blank document. Of course, a vendor won't tell you what is in their secret buffer, so I put in a help ticket and I asked - does this contain X-100, Tween-20 or any other scary detergents I should know about. And - yes - it contains a low amount of X-100.