Forwarding this one in just a second for sure!
Most of what I know about the HLA/MHC immunopeptidomics / neoantigen presentation system comes from this amazing old paper in Cell Immunity.
The mechanisms for processing and presentation are largely inferred from the large number of high confidence peptides they identify - but again - this is inference.
If we really wanted to understand how this system worked could you just knock out each protein along the way and do proteomics and immunopeptidomics? I mean, that's what a lot of people would tell you to do and that's what this cool new MCP paper does.
They knock out 11 proteins in the system and there are 3 or 4 that cause big systematic changes in what peptides are expressed on the cell surface. The ramifications are probably beyond me and absolutely beyond what time I've got to think about it today but some collaborators are going to totally dig all of this new insight!
No comments:
Post a Comment