Would you believe you could sample single cells directly off a plate and lyse/digest and transfer that cell with virtually no losses?
No? Sounds sort of unbelievable and there is some "extraordinary claims require some amazing evidence" or something.
Would you believe over 3,000 proteins per single cell with a TIMSTOF Pro 1 system running a 21 minute active gradient on those same cells?
No? Would that immediatley make you think -wow - that should have ultra super fucking amazingly extraordinary goddamned evidence cause I don't think I've ever gotten more than 500 proteins on a very similar system regardless of how long the gradient has been -even on a cell line I like a lot that has a higher protein content than either of those?
Would you be really annoyed that those instrument files weren't provided?
What if when you follow a bunch of links to the Electronic Supplemental Information where you were promised access to the data you instead find an Excel spreadsheet with 20 columns (10 cells each?) and 2150 rows for protein values and not one single missing value?
Would you start to wonder if there is actually something of a link between some groups and their ...unbelievable...advances in a new field and the fact they only seem to publish in places where providing your data isn't required? Maybe I'm just a jerk, but this study seemed so cool until about page 3.
I'm not linking the study, but if you see it, I'd suggest you crank the skepticism up to 11 or so.
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