Wow, am I super pumped to see this one out!
You know all those minimum handling techniques for single cells generally means that EVERYTHING is injected into the mass spectrometer, right?
This is a pretty minor alteration from my favorite paper of 2024 - CellenOne pulls out single cells, but instead of digesting them you drop in lipid standards and methanol and then run the TIMSTOF optimized for larger small molecules (lipids).
They have to do optimization in both positive and negative mode, the latter I've never even tried on a TIMSTOF unless I was doing MALDI, and then the entire screen changes. Fortunately, we don't have to do a darned thing, we'll just steal this method entirely.
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