Leaving this link here so I can find it. There is a point sometime when an ASAP JPR paper appears google searchable and may not make the ASAP page tab on the JPR website. Thanks @cportgrrl.bsky.social for helping me find it!
This straight up surprised the fuck out of me, btw. I assume when you dump in formaldehyde for any length of time you just stuck all your lysines together and globbed up the nucleotides and left oxidative secondary changes all over the place.
This does not appear to be the case! The authors take some HeLa cells and hit them with a DNA chelator (5-FU does that, I think, don't quote me, haven't used in years) and some other drug and looked at fixed vs. unfixed cells. You'll see some huge numbers in the early figures. 8,000 protein groups. That is from 1,000 cells digested in the top of an EvoTip at a time.
Protein groups drop a little with the brief formaldehyde treatment, but the differential proteins upon drug treatment look about the same - in some cases better - with formaldehyde rather than no treatment.
Does that mean you should quick formaldehyde your single cells? Maybe not - but maybe that would stabilize them if you can't analyze them immediately? Maybe that helps with shipping?
MORE IMPORTANTLY! Of the tens of thousands of samples I can access through my super cool new blanket IRB and hospital repositories at my university - about 80% of them are formalin fixed! I'm getting very bad ideas thanks to this really cool paper. I need to reproduce it first before I go spend money and time on said bad ideas, but - hot dog, yo, this could be huge.
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