How do ApoE isoforms affect protein turnover (for 4,900 proteins) in the mouse brain??

 

I missed this one with the move and never ending sickness 3 (now 4? eeeek!) year olds create when they climb all over each other in "class" and big open gyms. 

Thankfully it came up during the new US HUPO sponsored podcast mini-series "The Undergrad Expo" which highlighted shockingly capable undergraduates (which you might have gotten from context clues) and their research. 

If you want an intro, Noah Earls is the guy who brought it up and his podcast is a cool 30 short listen. My understanding is that there are 3 main isoforms of ApoE and one is good, one is neutral and one is really really bad. This team took mice with each isoform and deuterium labeled them (!!) and did biochemistry at a massive scale. For people who do ApoE research, I suspect the well curated Excel tables in the supplemental are going to be a really useful resource. 

The easiest method you've ever seen for profiling a large number of kinases fast!

 

The last time I was part of a study where we needed to understand what a bunch of kinases were doing, we sent samples out to 2 different labs. Some were measured fluorescence activated cell sorting (FACs) while the rest were ran by a group who had validated immunoprecipitation based QQQ assays. 

Now, in my defense, we couldn't go into the lab for a few months due to a serious flood that led to both mold and asbestos problems because....well... you don't go to Johns Hopkins because of the nice modern clean safe facilities.... But I didn't mind getting out of building all those targets myself. 

This group quantified 380 in the most streamlined procedure I've ever seen, and they get around a lot of up front development by using DIA (on an Exploris)! 

670,000 dose-response curves for KRAS inhibitors and combinatorials!

 

So....everything you ever wanted to know about KRAS inhibitors but didn't have the time to do 670,000 DOSE RESPONSE CURVES? 

Proteins, phospho-peptides (critical, cause these things screw with MAPK/mTOR/etc) and OTHER PTMS? Crazy.