This is a thoughtful addition to the challenges with carrier channel boosting proteomics samples. The isotopic impurities are something you can largely ignore when your signal is roughly equivalent, but when it isn't, it's a big problem.
It does make you wonder if there might be a way to make expensive multiplexing tags that are...I dunno.... actually pure...?
Is this a late April fool or are we genuinely supposed to believe that folks just woke up to this one?
ReplyDeleteand yet TMT dominated single cell proteomics for a long time. The multiplexing idea is still very prevalent.
DeleteIn our previous paper, we need to keep 7 TMT channels as empty to avoid the interference. https://www.nature.com/articles/s42003-022-04400-x (Figure 5)
ReplyDelete