This is big!
In our preprints we proposed what it would take to make LCMS a competitive diagnostic assay for the other SARS-CoV-2 assays out there. We know we probably can't beat RT-PCR, but we need to hit a magic number the ImmunoSwab assays and ELISA's and Protein Arrays can hit, because they've been validated to work!
That magic number is around a detection of 20 picograms viral protein/mL biological fluid.
And these people just showed you can hit that with PRM!
They used an Orbitrap Eclipse.
They used 60 or 90 minute gradients.
They used NanoLC.
You could argue none of these are ideal for clinical diagnostics (unless you're the manufacturer of the Orbitrap Eclipse, which, would probably argue that's a great idea at a little north of $1M USD per box), but as a proof of concept?!?! This is fantastic.
The files are at ProteomeXchange/PRIDE here.
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