Friday, December 21, 2012

Delayed fragmentation for improving reporter ions

This article is from 2011, but it had slipped past me until my friend Santosh brought it to my attention recently.  In the study, Savitski, et. al., study the effect of various settings of the chromatography function on an Orbitrap Velos on their number of quantifiable reporter ions.
Their results demonstrate that delaying the fragmentation of peptides bearing reporter ions, using optimal settings, can result in an increase in both identification and quantifiable peptides.  A surprise (to me) side effect is that they show a marked decrease in coisolation.  This is an extremely interesting proposal since other methods for reducing the coisolation of peptides in iTRAQ experiments cause a large increase in cycle time, where this one does not.

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