Saturday, December 29, 2012
2D isoelectric focusing
This month's JPR includes an article by Maria Pernemalm and Jahnne Lehtio at the Karolinska Institutet. The subject of the study is a new method for performing 2 dimensional isoelectric focusing. In the first dimension, proteins are separated by IEF. The strips are then cut, the proteins digested, and the resulting peptides are separated by IEF. The team reports a significant increase in their numbers of resolved proteins.
An advantage of this technique over that of the Offgel 2D system is that you don't have to buy the Offgel. The disadvantage is that normal IEF strips are a good bit more difficult to work with than the Offgel (IMHO).
A schematic of their technique is shown below.