There is a good bit of confusion out there about how to properly maintain Orbitrap instruments. This is natural, they are extremely complex instruments and there are now multiple generations of them. There has also never really been an official document that tells you clearly how to tune and calibrate each instrument.
The Achieving Peak Performance project is an attempt to rectify this. If you have a Velos based instrument (Orbitrap Velos, Velos Pro, or Elite) you can go to Planet Orbitrap now and download the document. It will give you step by step plans for optimizing your instrument to achieving the best accuracy and sensitivity in the least amount of time. Definitely check it out here.
Edit: I'm gonna refer back to this, and if you've got an Orbitrap with an S-lens you should download that document
These are the S-lens ideal settings:
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