The title of my post describes the paper pretty well. They used a combination of transcriptional and quantitative proteomics (iTRAQ) to interrogate some P.f. cultures that were treated with neuraminidase.
The iTRAQ proteomics samples were analyzed with a QStar Elite.
The highlight of the paper is the two very nice tables of the up- and down- regulated proteins found by iTRAQ (roughly 75-100) when the cells are treated with this agent.
While this paper is a nice solid study, it does a good job of highlighting how little we really know about this organism, as well as how relatively poorly the genes/proteins are currently annotated.