The newest issue of JPR contained the following article, you can read the abstract here.
The title is the lengthy: Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics.
I think it does a good job of addressing a question many scientists have considered -- why am I paying so much for sequencing grade trypsin, when trypsin is cheap in bulk.
The very thoroughly explored answer is in this paper. Lower quality (cheaper) trypsin = lower quality MS results. They examine the 6 different types of trypsin (and aren't afraid to list the manufacturer or distributor) and show what is good (which is fortunately what we buy!!!) and what leads to unassigned fragmentation spectra.
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