Saturday, March 11, 2017
Ready for a new PTM to worry about? Cysteine glycosylation is all over the place in human cells!
Fun fact: Did you know that O-GlcNAc modified proteins were discovered in Baltimore over 30 years ago? See, there's more to my home town than fantastic infrastructure and friendly people!
Glycoproteomics is kind of exploding right now -- the enrichments are better, the separations are better, and the mass specs are ridiculously better, and the software has almost caught up....and I wonder if this great new paper at ACS is just the tip of the iceberg....
A whole new class of glycopeptides right under our noses! The evidence looks pretty clear cut to me -- and first analysis from this group suggests that it isn't even rare. Once they had a mechanism to enrich and a pipeline to search for them in the data they report proteins with this modification in virtually every subcellular fraction!