Wednesday, May 20, 2026

Nanosplit the transcriptome and proteome from single cells (without the hard part!)

 


When I first saw this I thought - okay, so someone copied the nanosplits paper but they had an Asstral.

And it's almost what this is


...but nanosplits requires a technically tough step where you split the droplet containing your mostly lysed single cells. This protocol gets around that step. They still use the same silly robot to isolate the cells, but you absolutely don't need it here (where you basically do need it for nanosplits, it's tough to print that droplet array in a FACs core), and that's a huge win for anyone who doesn't have the slow silly robot. 

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