There used to be LCM(s) (laser capture microdissection thingies) everywhere! No joke, they almost died out to the point that one of the leading companies was briefly for sale at the price of a Baltimore/DC suburb house. At ABRF I looked at two very nice new ones and individual systems were in the very nice Pittsburgh house sort of price range.
MR-SP2 takes one of the legacy systems that you can't buy new anymore and optimizes it up for spatial proteomics with all the details you'd need to set it up yourself.
At 1-2 cell cuts they gett over 600 protein groups on a TIMSTOF Flex system equipped with an EvoSep. I had this exact same setup for ore than 2 years and I think my record is less than 400 proteins on a cancer cell. That might have been Whisper (100nL/min) 40SPD or maybe even 20SPD.
AND they get this out of FFPE tissue! Incredible approachable spatial proteomics without buying all brand new stuff.
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