Sometimes you just need a vacation from everything. I ambitiously downloaded a couple dozen papers to my kindle but I probably have only read half of them.
I spent some solid time on this new preprint for obvious reasons, though!
Anyone doing single cell proteomics (SCP) is probably running up against the same problems I am - like....are you sure this is the right technology for the problem? Are you sure it wouldn't be better to figure out a way to get 25 or 500 cells from that sample that are identical that way you can get thousands and thousands of proteins without trying very hard?
Here we seen an exploration of both large cell numbers (500 cells) and bulk homogenates and single cells. And here is the real kicker - even when we think we have the perfect marker for getting "identical" cells out of that population.
The coverage here is stupendous for these simple little cells. They may be getting as much as 50% of the total neutrophil proteome on this Astral and - yet again - we start to see important functional heterogeneity in systems that would be a whole lot easier to study if they'd just all do the exact same thing!
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