I did my PhD with an amazing chromatographer as a co-mentor. As such, it was just easier for him to handle all the hard stuff and I'd just run the vacuum chamber things. I like the simplest HPLC possible. Less to break and even better if I don't have to EVER look at a diagram like whatever the picture above is trying to explain to me. There are definitely switching valves involved.
However - I could sure think of 4 things I could use ultralow flow rates for right this second. What if I could get that with ONE loading pump by preloading plugs of solvent that naturally form gradients by diffusion? Sounds like magic, but I'm absolutely interested!
Check this craziness out here!
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