Friday, June 3, 2022

Double barrel 10uL/min TIMSTOF DIA -- 4k proteins in 15min!


Yikes. I might have to find something else to do because Proteomics seems to be growing up. CVs, data completeness, and instrument utilization (IU) measurements? 

IU is the actual amount of time that your instrument is collecting useful information. If you've got an EasyNLC it doesn't count that 15min to 7 hours that the pump is kind of thinking about whether it actually is at 0 bar before finally starting to flow solvent again. 

This group is getting 90% IU and phenomenal results by using 10uL/min flow rates and an extremely well defined double barrel chromatography setup. Totally worth checking out! 

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