Thursday, February 5, 2015
Why you should run your nanospray at lower voltage
This is pretty cool. You know, we always try to get our nanospray voltage to the lowest energy that will provide stable spray. Why wouldn't we just set it higher?
Well, I think this great picture I got to take yesterday at the University of Wisconsin illustrates the point. This is an EasySpray source running the same peptide (same scale) at different ESI voltages. Check out the oxidation peak that shows up when you crank up the electrospray voltage to 2.35kV! Crazy, right!?!
Thanks go to Greg for collecting this data and letting me share it!
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