Tuesday, February 10, 2015

Top tip -- fractionate your samples dozens of ways with your centrifuge

I just heard about this today.  Top Tip is a product from GlySci.com.  At first it seems kind of boring.  "Fractionate your peptides without an HPLC?  Big deal!"  Then you take a look at the number of chemistries you can use.

SCX?  Check
SAX? Check
WCX (whatever that is?)? Check!
Two kinds of IMAC? Double check

The list of available resins is seriously a page long.  You can check it out here.  I haven't used them, but they got a good endorsement from a guy who is probably better at this proteomics stuff than I am.  And I'd rather pipette or spin to fractionate than set up an offline HPLC fractionation!

No comments:

Post a Comment