Analysis of the proteomics data using spectral libraries has become possible but with the fact the unavailability of a comprehensive spectral library. Although, it is evident that such libraries will become more popular in coming years.
This technical note by Henry Lam Lab just accepted in JPR describes about the spectral library generation for phosphopeptides from human as well as four other model organisms. The claim is better sensitivity over conventional database searching to identify phosphorylated peptides. The other good part is that this library is made freely available to be searched using SpectraST algorithm (PD 1.4 has this algorithm in-build for spectral library searching).
I will be testing this phospho-spectral library and see how it performs in my hand.
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