Friday, October 11, 2013

Deep proteome coverage by 3 dimensional fractionation

Do you REALLY need proteomics depth?  I mean, do you REALLY need it?  They we know how to get it.  Fractionate fractionate fractionate.

This new paper from Ilian Atassov and Henning Urlaub shows how to get crazy deep proteome depth by employing a 3D separation approach.

1) Do SDS-page at the protein level
2) Cut band and digest
3) Separate the peptides by isoelectric focusing
4) Run those fractions on LC-MS/MS

Using this approach, they were able to identify >3,000 peptides out of a single gel slice.  Worth checking out if you really just have to get to the bottom of your proteome.

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