Monday, July 22, 2013

In-solution digestion -- the ultimate analysis


When looking at digestion protocols, have you ever stopped to wonder things like "is it 15mM because it was optimized, or is it 15mM because it worked okay and no one has changed it?"

If you have, do I ever have the paper for you!  In press at MCP right now is this paper, easily the most comprehensive study of in-solution digestion ever performed.  They didn't cut any corners here.  Thorough, thorough, thorough.

Later, when we wonder why we were using a certain concentration in a protocol, I hope everyone will just refer back to this paper (for in-solution) and others where there was no guesswork.

Highly recommended for anyone doing in-solution digestion, or who has done it in the past and found low efficiency.

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