Thursday, February 22, 2018
Structural prediction of protein models using distance restraints!
Amateur hour is over for structural proteomics, yo'. Time to take the formaldehyde and the guess work and get off the stage.
This is how you do it. Step by step. Reagents, mass spec settings, free software. Everything is in here. Okay -- actually -- you have to also have this paper (this is where the mass spec data came from) and THEN you have the entire workflow.
MS-Cleavable crosslinker was utilized (of course) but the gem here is the downstream analysis that takes you a step forward in your data. You go from this peptide is xlinked to this peptide to "holy cow this is the way this protein is folded or how these are linked".
As someone with a bunch of this planned in the near future -- this Protocol couldn't have come at a better time. Now I just need someone to install all this software....