During the pandemic I had no choice but to learn /relearn (had done it before, though poorly) how to grow cancer cells in culture. If you've never done it, you should check over the protocols to see what an ...art... it is... Which is what you never want to think about robust scientific workflows.
One of the findings of the best scientific work I've ever done was that the biggest difference between cancer cell lines I was studying with single cell proteomics was culture media additives. One cell line couldn't grow without insulin supplementation and the other could. Ultimately that difference convoluted any direct comparisons between drug effects in those cell lines.
For even more reasons to be concerned, check out this cool new JPR paper!
Also -- check out the impressive coverage they get of the HepG2 cells! >8,000 protein groups per condition in 30 min total run to run experiments (TTPro diaPASEF + DIA-NN 2.0). Solid study all around.
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