Did you know this was possible?!?!
Rather than metabolically label every protein in a cell like we normally do with SILAC or similar reagents, this group utilized cell-type specific promoters to only incorporate labels according to where they wanted them!
In this particular study they use this awesome technology to show what proteins change when mice are bored or when they have fun things to play with. The list is surprisingly large, but it is the promise of this technology that I find really exciting!
If you can label and enrich things as small and complex as different areas in mice brains -- accurately -- and you have cell-type specific promoters that are truly cell-type specific -- you can do loads of awesome stuff with this! Maybe settle some of these tumor vs. stromal cell/protein identifications once and for all, for example....
Downstream analysis was all done with a quadrupole Orbitrap (Plus) system and an LTQ Orbitrap Elite. Data analysis all appears to be MaxQuant/Perseus.
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