Can you see what that is? It is a way of diverting the sweep gas so it is useful for nanospray applications! At the beginning and end of a run, alternative tune files are used at BRIMS. These tune files contain instructions to turn on the sweep gas. This appears to effectively blow away the stuff that comes off the column at the beginning and end of the runs and extends the amount of time they have between cleanings!
Want to set your own up?
It's the P-430 Union at Upchurch Scientific. This setup has been shown to work on both LTQ Orbitrap systems and the Q Exactive.
Correction (thanks guys!) its a P432, not P430. For more details see this new post.
What pressure do you have to run the sweep gas at to prevent droplets from forming at the beginning and end of runs, but so that it doesn't interfere with nanospray?
ReplyDeleteCorrect me if I'm wrong, but it looks like a P-432 for 1/8" tubing. Anyway - seems like a useful method to keep you instrument going for longer without cleaning!
ReplyDeleteSweep gas settings of 1 do the trick. On instruments like the QE and Orbitrap you can segment the tune file, so that the sweep is off when things you want elute..
ReplyDeleteHi Ben and David,
ReplyDeletehow did you connect the tubing to the hole of the sweep gas?
Thanks.