Recently, I've heard of a number of a few groups who have had trouble with getting good peptide coverage using the FASP method. I'm still a big fan, but I realize that we might be trading reproducibility a little for sample digestion efficiency. There is obviously room, however, to improve on the FASP method and there are a lot of new variants out there.
In this month's Wiley Proteomics, Zougman et. al., go a completely different direction with their method, STrap. The method still seems relatively easy but maybe more robust than simple FASP. the article is open access (with Wiley registration) and worth checking out.
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