Tuesday, March 19, 2013
New article in press -- Identification of Protein Interactions Involved in Cellular Signalling
This new article is currently in press at MCP and comes to us from Westermarck et al., as a collaboration between groups in Turku, Finland at the Institutions whose emblems are shown above. This review takes a very critical look at the technologies currently available for interrogating cellular signaling networks. It goes after the classic techniques of the geneticist, such as the yeast two hybrid assay and systems such as strep- and flag- tags. They also take a look at affinity purification coupled MS, and tandem affinity purifications. You get a nice look at the strengths and weaknesses of each assay for studying cellular networks.