Preprinted data featured in Day 1 at SCP2026!

 

I've got to start a new page of notes for day 2 or OneNote will definitely crash. Obviously there is a lot of unpublished data here I don't know if I can share or not, but some of this is out or preprinted. 

If you very very carefully controlled pH and reaction kinetics could you label all of one side of a peptide with TMT and all of the other side with dimethyl labels? Apparently, yes.

Nikolai beat me to the question of "wait. better than 95% labeling efficiency ON BOTH TERMINI?"

The only link I can find to this publicly so far is a dissertation that is public (and really really well written) you can find here.

Now...the stochastic sampling appears to be a major issue because you have 3-plexes of each TMT tag. So you drop to a very low overlap from cell to cell. I'm personally about 90% sure that it's due to MIPs. That's a lot of complexity that is far outside of the model of averagine within a very tight m/z window, but it does still allow for separating very different cell types apart. They have tried semi-targeting and other intelligent acquisitions, but - this ain't a terrible start for huge throughput potential. Especially if they've worked out the double labeling kinetics so you and I don't have to. 

And there are always people at this meeting doing crazy stuff out there you never heard of, right? Like, where does Parallel Squared find these people? This was the first one of the meeting and it was preprinted a few months ago. 

Crazy and amazingly ambitious. So...what if you coupled expansion microscopy (where you put the diaper gel stuff on your tissue and stretch it out so you can image it? But you did that to de-crowd the spatial peptide domain enough that you could essentially do Edman single molecule degradation in a spatial context? You might immediately have objections about depth, complexity, and orders of magnitude or 6 that would be tough to reach, but - damn....I would have never ever thought of this, nor have I ever heard anything even remotely similar to this idea. Really really cool. 

One more. I don't understand this one, but it's another off-the-wall technology and I really liked the speaker and his animations for BALLISTIC MICROSCOPY!

MORE EMERIL! 

Day 2 is starting now! Let's go!