Back to the paper:
Another really good paper I'll be referencing all the time has done the carrier ratio suppression math and just to flag why I'm so impressed by them getting to the same conclusions:
We're a 20+ year old field and not one single one of us can digest a sample the same way. Proof in point?
Paper earlier this year?
1:50 trypsin with overnight digestion at room temperature
10mM DTT for 30 min at 56C
55mM IAA for 45 min at RT
Some other changes:
In Claudia's new paper we see something slightly different (surprise)
1:100 trypsin overnight at 37C
50mM DTT for 30 min at 37C
40mM IAA at room temp for 30 min