Thursday, May 16, 2013

IsobariQ: Use advanced variance statistics for reporter ion quan

I've been meaning to write this one for 6 months or so, but it always seems to slip down the list.  We're all aware of the limitations by now regarding reporter ion quan.  I've talked about some of them recently. A method of combating some of these limitations is a procedure called variance stablisation normalization (VSN).

While the maths are well beyond the capabilities of this biologists, the way my mathematically inclined friend Patricia explained it is that it uses clever statistics to re-evaluate the apparent ratios.  These magical formulas tend to improve ratio suppression and coisolation instances.

More tangible perks:  The software is free, appears well supported and commonly updated, and has a colorful and friendly looking user interface.

Still doing iTRAQ?  Maybe you should check this out!

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