Tailoring collision energies to search engines -- have be been too complacent?

On the large list of things that I thought we had settled and we, as a field, would never have to think about again.....

You should check out the evidence yourself here, the files are up on MASSIVE and the authors show solid arguments that for both QTOF and OrbitalTrap instruments we might want to think a little harder about how we set our collision energies....to the tune of as much as a 40% increase in identifications between optimal and the opposite of optimal.  The 40% looks like a pretty extreme example of sub-basement optimal,but it does help to highlight the importance of these parameters.