Sunday, July 17, 2011
Gallium enrichment of acetylated peptides
I've been spending the weekend reviewing some data from a while ago for something I meant to evaluate further. Pierce used to sell a phosphopeptide enrichment kit that used 'gallium swell disks' to capture and selectively elute phosphopeptides. When I first used this kit last year I remember being surprised by the number of acetylated peptides. Looking at the data further suggests that this kit may actually be better at enriching acetylated peptides than phosphorylated ones. Too bad they don't make it anymore, because it might be a nice tool if one were going after the acetylome.