There is another optTMT, but that one doesn't have a dash and it's for designing smart multi-batch mutiplexed experiments. You can read about that one here.
This new one is about how a lot of TMT labs are labeling 400 pounds of peptide (181 kg) and then injecting 200 micrograms per injection on their Orbitraps and 1000 micrograms on their Astrals.
If you wanted to just label 10x more peptide than you'd possibly use instead of 10 million times more peptide, how would you do it? That's what the dash is for!
While this might seem just a little silly since there are protocols out there that have been replicated dozens of times for labeling single human cells, they are actually a lot more convenient than you'd think. We know how much reagent in our lab to use for 1 cell or 25 cells and it's a drag when we have to break out the peptide quan kits and borrow someone's plate reader. This study gives you that in-between concentration fully optimized.
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