Y'all aren't going to believe this one. For real.
Everyone out there complaining about the number of proteins you can identify in plasma proteomics and no one has ever tried fractionating it??? What is wrong with us?
Whoops. Not everyone got that this is a joke. Okay...so...literally for all of time everyone has fractionated plasma in some way to get higher coverage. That makes the title funny. 15 years ago I was running SDS-PAGE gels and cutting fractions and running them separately on an LCMS. 13 years ago I was using something called an OFF-GEL to first fractionate the proteins at the intact level by isoelectric focusing and then digesting the proteins in those fractions and fractionating them at the peptide level to get proteomic depth. The problem with fractionating is that mass spec time is expensive and if each sample takes 144 hours to analyze (example) you only complete 60 samples per year if you never run a QC, a blank, your instrument never needs maintenance and you work every single day of the year. The UK Biobank study 1 would take 833 years. Most people aren't that patient and we're all sort of looking for ways to get a lot of samples analyzed before we retire.
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