Not sure if I'm allowed to show this yet - but - w0000000000000000000000000h00000000000000000!
I'm not kidding - I just put in a proposal for a 1 day workshop where this is the plan.
1) Participants are instructed to show up in the morning with something biological. I'm picturing flowers or pine needles or dirt.
2) We will homogenize that thing - prep with a high temp enzyme (FAST) to peptides
2a) We will find some way of estimating protein abundance fast (work in progress)
3) We'll load those samples onto EvoTips
4) At lunch we'll load those samples in my lab (or we'll have someone from my team drive them the 1.4 miles to my lab - work in progress, but the conference is here in Pittsburgh)
5) We'll process those data against whatever genomics data we have
6) Participants leave with a proteomic report of the thing they brought in.
If you know anyone at ABRF you should totally tell them they should do this workshop. 'Cause this is absolutely where I am today with LCMS proteomics. There is a beautiful vine growing on the dumpster at my apartment complex that the guys who do the landscaping here can't seem to kill and every day I walk by it I think I should take samples of the roots, leaves, stems, flowers and strangely complex fruiting bodies the plant has and do proteomics on it on a Saturday when an instrument isn't in use.
What I'm rambling about is the fact we did a black widow spider - without a good genome (though we got one later) - and could find multiple points of biological evidence that we have solid functional findings. Since it was a training excercise for a student it wasn't done in a day. But....at the end of his master's I'm pretty sure he could have.... (I tend to make new trainees prove they can do good proteomics on unlimited material before I let them anywhere near things like....patient biopsies...)
Huge shoutout to Ben Neely for his invaluable help with this project, btw. Dude solves proteomics of understudied organisms in his sleep.
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