For anyone out there who wants an Orbitrap to go super duper fast and are okay with getting less mass resolution than a Q-Trap in 2002 do I ever have an advance for you!
Introducing the FASTEST ORBITRAP EVER! All you have to do is ....give up....everything else....
...and run phase constraint!
Okay - so I'm obviously just being a jerk. I got in all sorts of trouble a while back for ...running some old instruments at whatever resolution I felt like and then putting funny stickers on them because I wanted faster scan speeds.
I won't draw attention to those posts -lawyers are expensive - but when we ran the instruments at higher scan rates we had two major problems
1) We weren't getting anywhere near enough signal for most molecules
2) The mass accuracy went out the window. Funny to say now because I was super appalled by 20-30ppm mass accuracy, and that's before I started buying TOFs...where that's pretty good...
What this group does is a bunch of magic to fix problem #1. By balancing accumulation times to get more they show they can get enough signal - EVEN at 8ms fill times to get good MS/MS spectra. Impressively, they get a solid number of IDs on an Exploris 480 running 5 - 8 minute active gradients. They get the best coverage at a whopping 500ng of peptides on those 8 minute gradients. Fortunately, you can clean that quadrupole yourself!
Now...how's the mass accuracy...? Better than an Ion Trap!
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