Increase your multiplexing by adding the time dimension (and more nanoLC columns!)

 

My first thought when I saw this new preprint was something like "I've been working on these IRB documents for my clinical trial for waaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaay too many hours, cause I can't figure this out at all." 

I honestly might still not get it, so the less I type the better, but this is probably the best figure for driving the idea home in the preprint. 

I do think it is an interesting idea, and I'm all about getting extra throughput. Multiple samples running with time delays are demonstrated as being deconvolute-able using retention time re-alignments. 

From a practical standpoint - 

Since 2020 I think roughly 30% of my nanoLC columns have arrived in a completely non-functional state. The ones above just weren't glued at the ends so the capillaries just fall right out at the fittings. 

There is a vendor that produces nanoLC columns that have been famously reliable and successful over those few years, but - in my hands- the columns are amazing for about 2 weeks before they overpressure.

The thought of relying on 3 separate nanoLC columns performing properly chronologically seems just about as likely as me winning the big Powerball jackpot thing by buying 3 tickets. I've never gambled in my life, but I'm assured the odds aren't great of that happening.