tag:blogger.com,1999:blog-3693238014525650573.post7755811583600120734..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Optimizing your nanoLC conditions part 4: The effects of a longer columnUnknownnoreply@blogger.comBlogger2125tag:blogger.com,1999:blog-3693238014525650573.post-23008736421992455472014-04-15T07:51:03.908-07:002014-04-15T07:51:03.908-07:00Santosh,
TFA ion suppression can be a pain in the...Santosh,<br /> TFA ion suppression can be a pain in the butt. Now, I'm pulling straight from memories dulled from time and a surprising number of head impacts for someone my age, but I think that the end goal was to keep the TFA levels below 0.3%. This can be tricky when using a desalting step that prefers a bunch of TFA. You have this scary middle ground where you are drying your sample down; are you simply concentrating the TFA or is it evaporating at the same rate? I'm not sure I've used SepPak, but for other desalting columns I've simply substituted TFA for formic acid. Man, I need to know more chemistry; but what I found was that the pH was really what seemed important. If you were acidified then peptides stuck. If any readers (who actually know some chemistry and about the C-18 acidic binding dynamics) it would be awesome if you'd commment here!Benhttps://www.blogger.com/profile/18385704761413487532noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-54764397047188718212014-04-02T23:31:38.583-07:002014-04-02T23:31:38.583-07:00I was just wondering regarding the use of TFA in m...I was just wondering regarding the use of TFA in mobile phase. I had problem of ion suppression when used it during Sep Pak (desalting step; washing and elution buffer). Ben, would you like to comment of this??Santoshhttps://www.blogger.com/profile/00444402763008219628noreply@blogger.com