tag:blogger.com,1999:blog-3693238014525650573.post7542615796467907006..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Iterative searching in Proteome Discoverer!Unknownnoreply@blogger.comBlogger2125tag:blogger.com,1999:blog-3693238014525650573.post-88459359764293955442013-10-15T19:03:22.454-07:002013-10-15T19:03:22.454-07:00Santosh,
Good question. Ultimately, the search e...Santosh,<br /> Good question. Ultimately, the search engines are going to disagree once in a while, especially when looking at lower res MS or MS/MS spectra. Due to the basic construction of Sequest (and we assume, Mascot as well, but no one really knows), if all things are equal between two spectral matches, the one with the PTM will still end up with a lower score. From a biological perspective it makes sense, as PTMs tend to have a low stoichiometry as compared to their non-modified counterparts. <br />-Ben Benhttps://www.blogger.com/profile/18385704761413487532noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-70852468073991598322013-10-13T06:13:11.016-07:002013-10-13T06:13:11.016-07:00Hi Ben,
I was wondering whether PD takes care of s...Hi Ben,<br />I was wondering whether PD takes care of same scan ID identified with different peptide with/without other modifications?<br />- Santosh<br /> Santoshhttps://www.blogger.com/profile/15244370709581433764noreply@blogger.com