tag:blogger.com,1999:blog-3693238014525650573.post5967516725002415788..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Somebody pulled off protein sequencing by nanopore! Unknownnoreply@blogger.comBlogger1125tag:blogger.com,1999:blog-3693238014525650573.post-8795298491660723202021-11-09T10:17:50.474-08:002021-11-09T10:17:50.474-08:00I've been enjoying this blog for a few years n...I've been enjoying this blog for a few years now, but I feel I have to step in to defend the humble (if poorly named) Flongle. You can do a lot with 500Mb-2Gb of long read data - especially if you're interested in sequencing a few bacterial or small eukaryotic genome, but don't have enough samples to make a full MinION worthwhile. And they only needed 211 reads to identify the peptide variants - I've gotten 100,000+ from a Flongle before. For ~70USD, being able to confidently identify a protein, including single amino acid variants, using mostly tools/supplies you probably already have in your lab, with a machine that doubles as a DNA sequencer? I can see that being very useful for anyone doing protein expression & modification, e.g. monoclonal antibodies.Anonymousnoreply@blogger.com