tag:blogger.com,1999:blog-3693238014525650573.post5769597206672311538..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Is there a difference between HPLC and LC-MS grade solvents?!?!?Unknownnoreply@blogger.comBlogger7125tag:blogger.com,1999:blog-3693238014525650573.post-3043275617846681262021-03-18T01:56:21.149-07:002021-03-18T01:56:21.149-07:00This comment has been removed by a blog administrator.Nitibha kaulhttps://www.blogger.com/profile/10712012076275609458noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-68331220006149445112021-02-15T22:41:24.369-08:002021-02-15T22:41:24.369-08:00This comment has been removed by a blog administrator.johnihttps://www.blogger.com/profile/05771688799725071256noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-8817222818472991032021-01-05T22:44:49.931-08:002021-01-05T22:44:49.931-08:00This comment has been removed by a blog administrator.lissa juddhttps://www.blogger.com/profile/00567711051113505685noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-64706506270646942052021-01-05T01:05:21.150-08:002021-01-05T01:05:21.150-08:00This comment has been removed by a blog administrator.lissa juddhttps://www.blogger.com/profile/00567711051113505685noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-69463537504152768682020-08-03T21:37:48.609-07:002020-08-03T21:37:48.609-07:00This comment has been removed by a blog administrator.Anonymoushttps://www.blogger.com/profile/15399758532752386750noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-31703518481921540252014-10-29T01:54:15.069-07:002014-10-29T01:54:15.069-07:00We've had issues with some ultrapure MS grade ...We've had issues with some ultrapure MS grade containing too high levels of sodium, potassium, calcium etc. - was very easy to see as peptide adducts in an LCMS run of BSA where peptides often elute as pure peptides (only 1 precursor mass should be present).Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-91500344931328508352014-10-22T02:18:58.081-07:002014-10-22T02:18:58.081-07:00My experience is actually the total opposite: I sy...My experience is actually the total opposite: I systematically tested methanol, acetonitrile and water in different grades (Ultrapure MS grade vs. HPLC grade or milliQ grade 3 in the case of water) and found no detectable background difference. The only difference I found was on the formic acid modifyer, where ultrapure formic acid gave around 25% lower baseline on mass transition in the lower mass range compared with crude formic acid of unknown grade. <br /><br />It may of course be that other analysts will find differences using their instr/methods, but my point is: test the solvents systematically using the same instrument (and of course the same timepoint) before making a choice of what to use as mobile phase.<br /><br />Cathrine, NTNUAnonymousnoreply@blogger.com