tag:blogger.com,1999:blog-3693238014525650573.post5353522094403908511..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Western blots versus parallel reaction monitoring (PRM)!!Unknownnoreply@blogger.comBlogger4125tag:blogger.com,1999:blog-3693238014525650573.post-6195839319107461272016-01-10T01:53:12.103-08:002016-01-10T01:53:12.103-08:00Err..perhaps explain how PRM can demonstrate that ...Err..perhaps explain how PRM can demonstrate that a protein has been fragmented? Also, PRM is not that sensitive, compared to the very best antibodies. <br /><br />The beauty of orthogonal methods is that they give different information. The problem with 'traditional' western blots is the stone-age technology. I'm hard core 'quant by MS' guy, but we have be running comparison of SRM (='PRM-gold' :-) ) with a commercial western blot system (no connection) and we note assay performances that are very similar, and that the quantitative western blot can detect proteins we cannot measure my MS.<br /><br />Lastly, the loss of connectivity elicited by proteolysis is a huge problem. We forget that we digest to harmonise chemical properties so that a gradient RP-HPLC run will work for most - that has never been feasible at the protein level. But, we really need to understand proteins, not peptides, and there is no method that resolves proteins better than 2-d PAGE - again, compromised by poor implementation rather than the intrinsic failing of the method. We should not conflate the two.<br /><br />Rob Beynon<br />Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-44987216426973826422016-01-08T18:22:06.623-08:002016-01-08T18:22:06.623-08:00Testify brother, testify!Testify brother, testify!Anonymoushttps://www.blogger.com/profile/06551803454093642609noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-3394168201733649552016-01-06T10:07:38.076-08:002016-01-06T10:07:38.076-08:00Nope, I just end up with so many things flagged as...Nope, I just end up with so many things flagged as misspelled on blogger that I can't even pay attention to them. Thanks for catching!Benhttps://www.blogger.com/profile/18385704761413487532noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-11335821612828459942016-01-06T08:20:06.573-08:002016-01-06T08:20:06.573-08:00Hi Ben, is it an American idiosyncrasy to write fe...Hi Ben, is it an American idiosyncrasy to write femto as fempto ? ;-) Stephen Osbornehttps://www.blogger.com/profile/06811684711202455766noreply@blogger.com